Off-Targeting of Base Editors: BE3 but not ABE induces substantial off-target single nucleotide variants
نویسندگان
چکیده
منابع مشابه
CRISPR/Cas9 systems targeting β-globin and CCR5 genes have substantial off-target activity
The ability to precisely modify endogenous genes can significantly facilitate biological studies and disease treatment, and the clustered regularly interspaced short palindromic repeats (CRISPR) systems have the potential to be powerful tools for genome engineering. However, the target specificity of CRISPR systems is largely unknown. Here we demonstrate that CRISPR/Cas9 systems targeting the h...
متن کاملAchieving Plant CRISPR Targeting that Limits Off-Target Effects.
The CRISPR-Cas9 system (clustered regularly interspaced short palindromic repeats with associated Cas9 protein) has been used to generate targeted changes for direct modification of endogenous genes in an increasing number of plant species; but development of plant genome editing has not yet fully considered potential off-target mismatches that may lead to unintended changes within the genome. ...
متن کاملONTARGET still OFF-TARGET?
Several large randomized, controlled trials have demonstrated that interruption of the renin-angiotensin-aldosterone system with angiotensin-converting enzyme inhibition (ACEi) or angiotensin receptor blockade (ARB) delays the progression of renal and cardiovascular disease.1–6 These beneficial effects of ACEis and ARBs are likely to be mediated through reductions in blood pressure and albuminu...
متن کاملONTARGET still OFF - TARGET ?
Several large randomized, controlled trials have demonstrated that interruption of the renin-angiotensin-aldosterone system with angiotensin-converting enzyme inhibition (ACEi) or angiotensin receptor blockade (ARB) delays the progression of renal and cardiovascular disease.1–6 These beneficial effects of ACEis and ARBs are likely to be mediated through reductions in blood pressure and albuminu...
متن کاملSingle base mismatches in the mRNA target site allow specific seed region-mediated off-target binding of siRNA targeting human coagulation factor 7
We have analyzed the off-target activity of two siRNAs (F7-1, F7-2) that knock-down human blood coagulation factor 7 mRNA. F7-1 modulates a significant number of non-target transcripts while F7-2 shows high selectivity for the target transcript under various experimental conditions. The 3'-UTRs of all F7-1 off-target genes show statistically significant enrichment of the reverse complement of t...
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ژورنال
عنوان ژورنال: Signal Transduction and Targeted Therapy
سال: 2019
ISSN: 2059-3635
DOI: 10.1038/s41392-019-0044-y